Regenerative Medicine Utrecht

Internships for Master students

Internship: Effects of bone marrow derived mesenchymal stromal cells and their secretome on injured intestinal epithelium

Allogenic hematopoietic stem cell transplantation (HSCT) is the treatment of choice for immunodefficiencies, bone marrow failure syndromes, lysosomal storage diseases and leukemia. Despite the steady progress that has been made in patient survival and quality of life, graft-versus-host disease (GvHD) is, next to infections, the major complication of the HSCT therapy with the 40 percent mortality rate. GvHD results from immunological attack on target recipient organs or tissues (such as the skin, liver and gut) by donor allogenic T cells that are transferred along with the allograft. In the last decade the use of mesenchymal stromal cells (MSCs) in the treatment of GvHD has received attention. MSCs can be isolated from bone marrow, adipose tissue, placenta, cord blood and other tissues 5. They have the ability to differentiate to adipocytes, chondrocytes, osteoblasts and myelosupportive cell lines. However, it is their immunoregulatory and regenerative properties, which make them such an attractive tool for cellular therapy. How MSC exert their beneficial effects in tissue injury and inflammation remains unclear. In the current project you will study the role of MSC and their secretome in the repair of intestinal tissue using as a model human organoids.

Techniques: Differential centrifugation and sucrose gradient, size exclusion chromatography, Western blotting, flow cytometry, cell culture, isolation of primary T-cells, confocal microscopy, nanoparticle tracking analysis, lentiviral transductions, co-culture, siRNA, shRNA knock down and Crispr-Cas9 techniques

The project is for minimum of 6 months and is a collaboration initiative of Dr. Magdalena Lorenowicz (expertise in MSC and MSC secretome) and Dr. Caroline Lindemans (expertise in GvHD).

Contact: Dr. Magdalena Lorenowicz; m.j.lorenowicz@umcutrecht.nl