Regenerative Medicine Utrecht

Internships for Master students

MSCs and MSC-derived exosomes – Coffer group

Immunosuppressive properties of MSC and MSC-derived exosomes.

Contact: Dr. Magdalena Lorenowicz (m.j.lorenowicz@umcutrecht.nl)

Mesenchymal stromal cells (MSC) form a heterogeneous population of plastic-adherent cells that can be isolated from bone marrow, adipose tissue, cord blood and other sources. They have the ability to differentiate to adipocytes, chondrocytes, osteoblasts and are used as stromal cells to support in vitro maintenance of primitive hematopoietic cells. However, it is their immunoregulatory, regenerative and trophic properties, which make them an attractive tool for cellular therapy in alloimmunity, autoimmunity and inflammation. How MSC exert their immunosuppressive effects in tissue injury and inflammation remains unclear. In the current project you will study the role of MSC-derived extracellular vesicles in the immunosuppressive properties of MSC.

Techniques: Differential centrifugation and sucrose gradient, Western blotting, flow cytometry, cell culture, isolation of primary T-cells, confocal microscopy, electron microscopy, nanoparticle tracking analysis, cutting edge cloning techniques, lentiviral transductions, making transgenic cell lines, co- culture, siRNA and shRNA knock down techniques

The project is for minimum of 6 months.
Contact: Dr. Magdalena Lorenowicz (m.j.lorenowicz@umcutrecht.nl)